Real-time PCR

Folder qTOWER 2.0

The qTOWER 2.0 allows the use of standard consumables and the inclusion of up to 6 color and FRET modules.

With state-of-the-art heating rates of up to
5.5 °C/s and cooling rates of up to 4 °C/s the overall system in block 96'er format is optimally prepared to all common real-time applications.

The qTOWER 2.0 has a patented fiber-optic system for detecting fluorescence signals in a homogeneous sample volumes of 10-60 µl.

Beside the qTOWER for real-time rapidPCR, the qTOWER 2.0 allows the application of 0.2 ml standard consumables and the usage of up to 6 Color or FRET modules.

 

 

 

 
  • 5.5 °C/sec heating and 4°C/sec cooling
  • Optimized for standard sample consumption of 10 – 60 µl
  • Patented, fiber-optic system
  • Usage of all common 0.2 ml plastic ware (tubes, strips, microplates)
  • Maximum of 6 color channels for flexible adaption and multiplexing
  • Choice out of 10 excitation and emission filters of the different Color and FRET modules (anytime upgradable)
  • High-speed fluorescence scan in just 6 seconds
  • All common analysis methods included
  • Multilingual software
 
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The qTOWER, a newcomer on the real-time market, sets new standards for speed.

Based on the established rapidPCR, the qTOWER is up to 10 times faster than commonly available systems, achieving heating rates of 12 °C/sec and cooling rates of 8 °C/sec.

Completely quantitative PCR runs can be performed in less than 25 min. The significant reduction of reaction volumes (down to 5 µL) is yet another highlight, as is the exceptional savings (up to 75 %) of expensive real-time reagents.

 

product video

 

 

 
  • Quantitative real-time fluorescence detection
  • Combines advantages of rapidPCR with enormous sample reduction
  • Heating rates of up to 12 °C/sec and cooling rates of up to 8 °C/sec
  • Low sample consumption down to 5 µl
  • 9 different color modules available, including 4 FRET filters
  • Open for individually adaptations or changes
  • Read-out of 96 wells within 4 sec, independent of the number of dyes
  • Integrated control and analysis software qPCRsoft
  • Real-time PCR and analysis of 96 samples in parallel within less than 60 minutes
  • Variety of methods for data analysis
  • Absolute and relative quantification

 

The integrated SPS (Sample-Protection-System) also provides optimum sample protection within the thermal block, which is cooled down to 20 °C while the lid heats up to 120 °C prior to starting the actual PCR. The adjustable lid temperature and high contact pressure results in nearly 100 % sample recovery. In addition, condensation effects can also be avoided for small reaction volumes.

 

Impressive flexibility

The patented fiber-optic system at the heart of qTOWER guarantees detection of homogenous fluorescence signals across the whole microplate. The qTOWER can be equipped with up to four different measuring channels, which makes the device very flexible and adaptable for various applications. The user can choose from nine high-resolution qPCR excitation and emission filters (Color and FRET modules).As a result, the qTOWER is capable of performing ambitious multiplex analyses and covers a broad range of commonly used fluorescence dyes. In addition, the exceptional scan speed of the plate is impressive, because one 96 well microplate will be read out in just four seconds, regardless of the number of colors measured.

 

qPCRsoft – simple and intuitive

The integrated, intuitive qPCRsoft software serves as the foundation for the final analysis of real-time PCR curves. The program automatically generates different methods for evaluating measured fluorescence data. The program can determine PCR efficiencies and perform absolute and relative quantifications, as well as the delta-delta Ct method and allele discrimination (among other techniques). Researchers can use qPCRsoft to investigate reliable concentrations and precise allele conditions and to display exact expression ratios. Once defined, parameter sets can be applied as templates for future applications and be reused continuously.

Intuitive, exceptionally fast and easy-to-use qPCRsoft controls not only rapid? PCR runs and detects fluorescence signals, it also uses various qPCR methods for evaluating the final data.It follows that qTOWER and the corresponding software combine to form an excellent, highly flexible and exceptional fast real-time rapidPCR system that truly leaves nothing to be desired.

 

Initiative for energy efficiency

No environmentally hazardous substances, such as lead, mercury, cadmium, hexavalent chromium, PBB or PBDE, were used during the production of qTOWER. The qTOWER also stays ahead of the pack in terms of energy con- sumption. Up to 23 times more efficient than competing models, the qTOWER can dramatically reduce both costs and CO2 emissions.Go green and earth friendly: qTOWER – quantitative real-time rapidPCR.