Reagents for Standard PCR

Here you find our product range for PCR reagents which are used when performing a standard PCR. We are offering a wide range on polymerases, ladders, dNTPs and as well RT-enzymes.

 

Pipette

Analytik Jena‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ - 3’ DNA polymerase activity. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C). It is recommended for use in routine PCR reactions.

 

Concentration*: 5 U/µl

Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% Nonidet P40, 50% Glycerol

PCR Buffer: 10x KCl reaction buffer, without MgCl2, 10x (NH4)2SO4 reaction buffer, without MgCl2

Mg2+ solution: MgCl2 stock solution, 25 mM

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-EZ-1000500 500 units


*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs
into a polynucleotide fraction in 30 minutes at 70°C.

 

innuTaq RED DNA Polymerase has a special formulation, which contains a non-toxic and non-hazardous red dye. The strong red color also provides an easy and quick check of reactions to which the enzyme has been added, facilitates the confirmation of complete mixing, making it very suitable for standard applications.
The reaction products are ready for direct gel loading and the dye serves as marker for electrophoresis progress monitoring. The presence of the dye has no effect to the application. Purified from an recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5`- 3` DNA polymerase activity. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C).

 

Concentration*: 1 U/µl

Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% Nonidet P40, 50% Glycerol

PCR Buffer: 10x KCl reaction buffer, without MgCl2, 10x (NH4)2SO4 reaction buffer, without MgCl2

Mg2+ solution: MgCl2 stock solution, 25 mM

Store at –20°C, avoid frequent thawing and freezing

 

Order-No.
 Amount
845-EZ-2000500 500 units


*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs
into a polynucleotide fraction in 30 minutes at 70°C.

 

innuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5‘ - 3‘ direction in the presence of magnesium. It also possesses a 5‘ - 3‘ polymerization-dependent exonuclease replacement activity but lacks a 3‘ - 5‘ exonuclease activity. innuTaq HOT-A DNA Polymerase requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.

 

Concentration*: 5 U/µl

Enzyme storage buffer: 20 mM Tris-HCl, 100 mM KCl, 0.1 EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% Nonidet P-40, 50% (v/v) Glycerol, pH 8.0 (25°C)

PCR Buffer: 10x Hotstart Buffer complete: 200 mM Tris-HCL, 500 mM KCl, 15 mM, MgCl2, pH8,5,
10x Hotstart Buffer without MgCl2: 200 mM Tris-HCL, 500 mM KCl, pH8,5

Mg2+ solution: MgCl2 stock solution, 25 mM

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-EZ-3000500 500 units


*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs
into a polynucleotide fraction in 30 minutes at 70°C.

 

Long Range Taq DNA Polymerase is a mixture of recombinant thermostable Taq DNA polymerase with a second thermostable polymerase. It is optimized for amplification of long DNA templates. Using Long Range Taq DNA Polymerase, a routine extension is possible with significantly better fidelity compared to other standard Taq Polymerase.

 

Concentration*: 5 U/µl

Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA,
0.5% Tween 20, 0.5% Nonidet P40, 50% Glycerol

PCR Buffer: 10x KCl reaction buffer, without MgCl2, 10x (NH4)2SO4 reaction buffer, without MgCl2

Mg2+ solution: MgCl2 stock solution, 25 mM

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-EZ-4000500 500 units


*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs
into a polynucleotide fraction in 30 minutes at 70°C.

 

innuTaq TITAN DNA Polymerase is a highly processive, thermostable DNA polymerase. Due to its genetic modifications the innuTaq TITAN DNA Polymerase has an enhanced stability at room temperature with no activity loss up to 1 month. The enzyme has a 5‘ - 3‘ polymerization-dependent exonuclease replacement activity but lacks a 3‘ - 5‘ exonuclease activity. It is recommended for a wide range of PCR assays and TA cloning.

 

Concentration*: 5 U/µl

Enzyme storage buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol and stabilizers

PCR Buffer: 10x (NH4)2SO4 reaction buffer, with MgCl2, 10x (NH4)2SO4 reaction buffer, without MgCl2 and detergent-free

Mg2+ solution: MgCl2 stock solution, 25 mM

10x Solution S: additive, that facilitates amplifications of difficult templates (e.g. GC-rich DNA templates)

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-EZ-8000050 50 units
845-EZ-8000500 500 units


*One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs
into a polynucleotide fraction in 30 minutes at 70°C.

 

innuScript Reverse Transcriptase is tested by generating cDNA from various RNA templates, with products ranging from 0.5 to 9.0 kb in size.

 

innuScript Reverse Transcriptase is a genetically engineered version of MMLV reverse transcriptase that exhibits improved specific activity over a broad range of cDNA synthesis temperatures from 42°C to 55°C. In addition to being more versatile compared  to other reverse transcriptases, innuScript Reverse Transcriptase produces higher cDNA yields and provides superior performance in RT-PCR.

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-RT-5000010 10 reactions
845-RT-5000050 50 reactions
845-RT-5000200 200 reactions
 

A set of 4 separate 100 mM solutions (dATP, dGTP, dCTP, dTTP). Each tube contains 25 µmol (250 µl) of the corresponding dNTP. Quality control: The desoxynucleotide solutions have a purity of > 98% and are function-
ally tested by real-time amplification of 30 kb DNA fragments.

 

Pack size: 4 x 25 µmol

Concentration: 100 mM of each dNTP

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-AS-9000002 4 x 0.25 ml

 

 

Analytik Jena‘s ready-to-use nucleotide mix provides highest quality of desoxynucleotides. All dNTPs are ultra pure (> 98%) and quality checked by a set of PCR, RT-PCR and Klenow reactions. It is supplied as a 50-fold concentrated mix of ultra pure dATP, dCTP, dGTP, and dTTP with 12.5 mM each. The total amount of dNTP in each tube is 25 µmol (6.25 µmol of each dNTP).Quality control: The desoxynucleotide solution has a purity of > 98% and is functionally tested by real-time amplification of 30 kb DNA fragments.

 

Pack size: 2 x 25 µmol

Concentration: 12.5 mM of each dNTP

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-AS-9000001 2 x 0.5 ml
 

The innuSTAR DNA Ladder has been developed for precise sizing of DNA fragments from 100-1000 bp on agarose gels. This DNA Ladder has been compared by our customers with several ladders currently in the market. The Ladder is stable and reliable with easily distinguishable bands.

 

Features of the ladder:

  • 100 lanes per tube
  • Stable at 4°C for 6 months
  • Free of residual NTPs
  • Available as conventional and Express format
  • Express can be loaded directly onto gels
  • Consists of multiple repeats of 100 bp fragments to be easy remembered
  • Bands stain uniformly in ethidium bromide; lower bands appear weaker in agarose gels under 2%


Volume: 500 µl per tube

Concentration: 100 ng/µl

Recommended loading volumes: 5 µl

Store at -20°C for long term stability, avoid frequent thawing and freezing

Order-No.
 Amount
845-ST-1010100 500 µl
845-ST-1010500 5 x 500 µl
 
 
Order-No.
 Amount
845-ST-3010006 6 x 1 ml


Further Information will follow soon.

 
 
Order-No.
 Amount
845-ST-4010006 6 x 1 ml


Further information will follow soon.

 
 
Order-No.
 Amount
845-ST-1010100 500 µl
845-ST-1010500 5 x 500


Further information will follow soon.

 

A 25 mM MgCl2 solution for polymerase chain reaction. The amount of MgCl2 has to be optimized in dependance of other PCR conditions (Taq polymerase, primer, template etc.).

 
Order-No.
 Amount
845-AS-1000015 3 x 1.5 ml


Further information will follow soon.

 

5x SuperAmp Additive is a heat-stable and chemically inert low molecular weight compound, extracted and purified from thermophilic microorganisms. By stabilizing the native conformation of enzymes and enzyme complexes in vitro, 5x SuperAmp additive provides a higher product yield and lower background.
5x SuperAmp improves the thermostability of thermophilic polymerases and increases the half-life of many other enzymes in vitro. 5x SuperAmp additive also prevents biomolecules from premature denaturation under thermal or other stress conditions. In DNA polymerase assays 5x SuperAmp Additive suppresses the accumulation of false-primed fragments, mismatch extensions and concatemers. 5x SuperAmp Additive is very easy to use. No template/primer specific concentration optimum needs to be determined. 5x SuperAmp additive is compatible with any pre-optimized incubation buffer/additive composition. Unlike other buffer additives (DMSO, formaldehyde) it is stable and inert and does not affect the structural integrity of the enzymes or templates used.

 

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-AS-6000010 1.0 ml
 

A 10-fold concentrated Taq Polymerase Buffer for PCR. This buffer is optimized to improve amplification rates of a PCR amplification process, especially for longer and difficult templates. The buffer can also be used together with all DNA polymerases from Analytik Jena | bio solutions.

 

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-AS-7000015 3 x 1.5 ml
 

A 10-fold concentrated Taq Polymerase Buffer for PCR. The buffer can also be used together with all DNA polymerases from Analytik Jena | bio solutions.

 

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-AS-8000015 3 x 1.5 ml
 

The PCR ready mix is a PCR master mix including an optimized PCR buffer, Mg2+ amount and dNTP‘s (dATP, dCTP, dGTP, dTTP). The master mix offers a simple workflow and faster turnaround. It is suitable for researchers who want to save time and increase sample throughput by using standard PCR thermal cyclers.
 The delivered amount of PCR ready mix is sufficient for 100 amplification reactions à 15 µl or 60 amplification reactions à 25 µl.

 

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-RM-2001000 For 100  –  200 amplification reactions
 

The PCR ready mix plus is a PCR master mix including an optimized PCR buffer, Mg2+  amount and dNTP‘s (dATP, dCTP, dGTP, dTTP). The PCR ready mix plus contains also a DNA Polymerase. The master mix offers a simple workflow and faster turnaround. It is suitable for researchers who want to save time and increase sample throughput by using standard PCR thermal cyclers. The delivered amount of PCR ready mix plus and innuTaq DNA Polymerase is sufficient for 100 amplification reactions à 15 µl or 60 amplification reactions à 25 µl.

 

Store at –20°C, avoid frequent thawing and freezing

Order-No.
 Amount
845-RM-2101000 For 100  –  200 amplification reactions